TPP Demo2009 - 7. Visualize LC-MS/MS data using Pep3D 使用 Pep3D 來瀏覽 LC-MS/MS 圖譜

Pep3D is a tool for visualizing LC MS data, along with results from PeptideProphet.

Pep3D 是用來視覺化 LC MS 資料的工具，可搭配 PeptideProphet 的結果檔。

• Under the Utilities -> Browse Files section in Petunia, navigate to the demo2009\tandem directory. (nB. you may already be in that directory.) You may also select the interact.pep.xml file under the spectrast folder.

將滑鼠移至上方的"Utilities" 選項，並從跳出的視窗中，選擇"Browse Files"選項。

• Open the PeptideProphet results file by clicking on the [ PepXML ] link next to the file named interact.pep.xml. This will launch the PepXMLViewer application.

點選 interact.pep.xml 右方的 PepXML 連結以打開 PepXMLViewer。

• Click on the Other Actions top-level tab, and then on the Generate Pep3D button. A new window will launch the Pep3D viewer.

點選"Other Actions"標籤，再按下"generate Pep3D"按鈕"即可開啟 Pep3D viewer。

• Leave the default options (or change to taste) and click on the Generate Pep3D Image button.

保留預設值，直接按下"Generate Pep3D Image"按鈕。

• After a few moments, you should see two images displayed on the page, one per mzML input file. IMG:Pep3D

等待完成後，即可看到該結果檔所包含的2個 mzML 圖譜資訊。